Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites / 中华医学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.</p><p><b>METHODS</b>Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.</p><p><b>RESULTS</b>Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.</p><p><b>CONCLUSION</b>These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.</p>