Detection of Axillary Lymph Node Micrometastases in Breast Cancer Using RT-PCR Comparison the Results of MUC1, Cytokeratin 19 / 한국유방암학회지
Journal of Korean Breast Cancer Society
; : 154-160, 2002.
Article
en Ko
| WPRIM
| ID: wpr-28235
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: The detection of axillary lymph-node micrometastases in breast-cancer patients by using the reverse transcriptase-polymerase chain reaction (RT-PCR) may provide a good guide for postoperative therapy. To evaluate effectiveness of RT-PCR for the detection of micrometastasis, we have compared the results of conventional, immunohistochemical (IHC) staining with those of RT-PCR. Breast cancer, Lymph node micrometastases, MUC1, Cytokeratin 19, RT-PCR ] METHODS: We conducted RT-PCR amplifications of MUC1 and cytokeratin (CK) 19 on lymph nodes from 40 breast- cancer patients and conducted IHC staining for cytokeratin. The results of histological examination and cytokeratin IHC staining were compared with the RT-PCR results for the detection of lymph-node micrometastases. RESULTS: Nineteen of 40 cases (47.5%) were lymph-node positive and twenty-one cases (52.5%) were lymph-node negative. Using RT-PCR MUC1 and CK19 expressions were detected in all positive lymph nodes and in 4 (19.0%) and 5 (23.8%), in negative nodes, respectively. Not all positive nodes were stained by IHC (94.7%), and none of the negative lymph nodes were stained by IHC. Thus, IHC staining was ineffective in detecting micrometastases. CONCLUSION: Detection of MUC1 and CK19 by using RT- PCR can be a more accurate and useful method than IHC staining for the detection of axillary lymph-node micrometastases in breast cancer.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Mama
/
Neoplasias de la Mama
/
Reacción en Cadena de la Polimerasa
/
Queratina-19
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Micrometástasis de Neoplasia
/
Queratinas
/
Ganglios Linfáticos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Ko
Revista:
Journal of Korean Breast Cancer Society
Año:
2002
Tipo del documento:
Article