Determination of fumonisin biosynthesis gene and toxicity of Fusarium moniliforme strains isolated in China / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 249-252, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-282353
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between fumonisin biosynthesis gene and toxicity of Fusarium moniliforme isolated in China.</p><p><b>METHODS</b>The toxigenic gene of 29 Fusarium moniliforme isolated from different provinces and varied food samples were determined. Eighteen fum5-positive strains were selected for biosyhesizing fumonisin and determined by high performance liquid chromatography (HPLC).</p><p><b>RESULTS</b>Twenty-six isolates were identified as fum5 gene positive strains. And all of these strains produced FB(1), FB(2) and FB(3). The amount of FB(1), FB(2) and FB(3) was ranging from 0.41-140.20 mg/kg, 0.06-14.30 mg/kg to 0.30-58.00 mg/kg, except one strain produced a lower level of FB(1) only. It wight be the first report showing a high level fumonisin-producing strain isolated from the sesame sample and identified in the world. The amount of FB(1), FB(2) and FB(3) produced by the isolate was 128.84 mg/kg, 11.80 mg/kg and 14.88 mg/kg.</p><p><b>CONCLUSIONS</b>It should have a close relationship between fumonisins biosynthesis gene and toxicity of Fusarium moniliforme isolated in China. The study demonstrated that strain of Fusarium moniliforme might contaminate the sesame sample and produce a high level of fumonisins.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Fúngicas
/
Contaminación de Alimentos
/
China
/
Cromatografía Líquida de Alta Presión
/
Sesamum
/
Fumonisinas
/
Fusarium
/
Genética
/
Metabolismo
/
Microbiología
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Chinese Journal of Preventive Medicine
Año:
2005
Tipo del documento:
Artículo
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