Effects of dexamethasone on intracellular expression of Th17 cytokine interleukin 17 in asthmatic mice / 南方医科大学学报
Journal of Southern Medical University
; (12): 1185-1188, 2009.
Article
en Zh
| WPRIM
| ID: wpr-282586
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of dexamethasone on intracellular expression of Th17 cytokine interleukin 17 and the mechanisms in asthmatic mice.</p><p><b>METHODS</b>Experimental asthma was induced by ovalbumin (OVA) sensitization in 20 in female Balb/c mice with (dexamethasone group, n=10) or without dexamethasone treatment (model group, n=10), with another 10 serving as the control group. The levels of IL-17 in the bronchoalveolar lavage fluid (BALF) and serum of the mice were measured by enzyme-linked immunosorbent assay (ELISA), and the airway inflammation was evaluated by HE staining. The expressions of IL-17 and RORgammat mRNA were measured by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of RORgammat protein was measured by immunohistochemical staining.</p><p><b>RESULTS</b>The levels of RORgammat and IL-17 mRNA and protein in the asthmatic model group were significantly higher than those in the control group (P<0.01), and the increased expressions of RORgammat and IL-17 mRNA and protein in the asthmatic mice were significantly reduced by dexamethasone treatment (P<0.05).</p><p><b>CONCLUSION</b>Dexamethasone can inhibit the release of IL-17 probably by inhibiting RORgammat expression and blocking Th17 differentiation in asthmatic mice.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
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Asma
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ARN Mensajero
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Dexametasona
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Ovalbúmina
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Subgrupos de Linfocitos T
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Linfocitos T Colaboradores-Inductores
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Interleucina-17
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Alergia e Inmunología
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Miembro 3 del Grupo F de la Subfamilia 1 de Receptores Nucleares
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Article