Application of bioinformatics analysis of signal peptide in the identification of Neurospora crassa phyA gene / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1098-1101, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-282611
ABSTRACT
<p><b>OBJECTIVE</b>To identify the function of the gene encoding Neurospora crassa EAA33149.1 protein which has 46.85% similarity with Aspergillus niger phA gene.</p><p><b>METHODS</b>The bioinformatics analysis was conducted using the prediction algorithms SignalP v3.0, arginine and lysine propeptide cleavage sites in eukaryotic protein sequence prediction algorithms ProP 1.0 server, transmembrane domain prediction algorithms Tmpred and TMHMM v2.0, potential GP I-anchor sites prediction algorithm big-P I Predictor and the subcellular protein location prediction algorithms TargetP v1.01. According to the analysis results, the gene was cloned into Saccharomyces cerevisiae.</p><p><b>RESULTS</b>The signal peptide, the cleavage site and the secretion pathway were determined, and the expressed recombinant protein with 54,000 displayed phytase activity.</p><p><b>CONCLUSION</b>The gene has been identified to encode N. crassa phyA.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Saccharomyces cerevisiae
/
Algoritmos
/
Proteínas Recombinantes
/
Proteínas Fúngicas
/
Datos de Secuencia Molecular
/
Secuencia de Aminoácidos
/
Biología Computacional
/
Fitocromo A
/
Genética
/
Metabolismo
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS