Molecular basis of Rh DEL phenotype in Zhejiang Han population / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1029-1032, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-282738
ABSTRACT
This study was purposed to investigate the molecular basis of Rh DEL phenotype. Rh DEL phenotypes were identified by a serologic adsorption-elution method, the nucleotide sequences of ten RHD exons and exon-intron boundary regions were evaluated by a RHD gene-specific PCR-SSP (PCR-SSP, polymerase chain reaction-sequence specific primer) and sequencing. The results showed that out of 122 random Rh negative donors 35 Rh DEL phenotypes were identified through serologic method, including 6 RhCCdee (17.14%), 28 RhCcdee (80.00%), and 1RhCcdEe (2.86%). Sequence analysis indicated that all DEL phenotypes harbored a RHD 1227 G > A mutation in exon 9. D zygosity test revealed that 29 DEL phenotypes (28 RhCcdee and 1 RhCcdEe) had one RHD gene deleted, and 6 DEL phenotypes (6 RhCCdee) had homogenous RHD gene. It is concluded that RHD 1227A is an important genetic marker for Rh DEL phenotype in Zhejiang Han population.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenotipo
/
Polimorfismo Genético
/
Sistema del Grupo Sanguíneo Rh-Hr
/
Donantes de Sangre
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
China
/
Reacción en Cadena de la Polimerasa
/
Exones
/
Análisis de Secuencia de ADN
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2006
Tipo del documento:
Artículo
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