Isolation and physiological characterization of mesenteric arterial smooth muscle cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 954-958, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-282877
ABSTRACT
Vascular smooth muscle cells (VSMCs) are crucial for studying the mechanism and regulation of vasogenic response, but so far no ideal method has been reported for their isolation. We established a VMSC isolation method based on two-step digestion, with which a large population of single smooth muscle cells could be obtained. After cell isolation, the potassium current, cytosolic calcium concentration, reactivity to caffeine were measured, and the results demonstrated that the cells were highly viable and exhibited reactivity to the vascular constrictor to meet various needs in experimental research.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Canales de Potasio
/
Separación Celular
/
Calcio
/
Ratas Wistar
/
Técnicas de Placa-Clamp
/
Biología Celular
/
Miocitos del Músculo Liso
/
Potenciales de la Membrana
/
Arterias Mesentéricas
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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