Resveratrol promotes Ca2+-induced Ca2+ release from rat liver cell mitochondria mediated by Ca2+ / 南方医科大学学报
Journal of Southern Medical University
; (12): 910-913, 2006.
Article
en Zh
| WPRIM
| ID: wpr-282887
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of resveratrol (Res) on mitochondrial opening and Ca(2+)-induced Ca(2+) release (CICR) from rat liver cell mitochondria mediated by Ca(2+).</p><p><b>METHODS</b>Wistar rat liver cell mitochondria was extracted and Res-induced mitochondrial swelling was assessed spectrophotometrically at 540 nm to examine the permeability transition pore (PTP) opening. The membrane potential changes of Res-treated mitochondria were measured with fluorescence spectrophotometery. Ca(2+) uptake and release by the mitochondria was determined by absorbance change of arsenazo III at 685-675 nm monitored by dual wavelength spectrophotometry.</p><p><b>RESULTS</b>Res promoted Ca(2+)-mediated PTP opening, and this effect was completely inhibited by CsA and lowered by trifluoperazine. CICR accelerated by Res treatment was completely blocked by ruthenium red and partly by trifluoperazine.</p><p><b>CONCLUSION</b>Res can promote PTP opening by inducing CICR, which may be one of the pathways that Res induces cell apoptosis.</p>
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Asunto principal:
Farmacología
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Estilbenos
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Mitocondrias Hepáticas
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Células Cultivadas
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Calcio
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Ratas Wistar
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Hepatocitos
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Biología Celular
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Proteínas de Transporte de Membrana Mitocondrial
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Article