Effect of cell surface sialic acid and their linkages on adhesion of mammary carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 742-746, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-282928
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cell surface sialic acid and its linkage on the cell-cell and cell-matrix adhesion of mammary carcinoma cells MD-MB-435.</p><p><b>METHODS</b>MD-MB-435 cells were sense-transfected with ST6Gal I cDNA or antisense-transfected with part of the ST6Gal I sequence inserted in pcDNA 3.1 vector, with mock transfection with pcDNA3.1 vector as the control. The cell surface alpha2, 6-linked sialylation was determined by fluorescence-activated cell sorting (FACS) using lectin SNA (Sambucus nigra agglutinin specific to alpha2, 6-linked sialic acid on N-linked glycoprotein). A significantly increased alpha2, 6-sialylation subclone in sense-transfectants and a decreased alpha2, 6-sialylation subclone in antisense-transfectants were selected for further examination of cell-cell and cell-matrix (collagen IV) adhesion. The transfectants were also treated with sialidase to compare the capacity of cell adhesion affected by cell surface sialylation.</p><p><b>RESULTS</b>Sense-transfection subclone showed a reduced cell-cell aggregation but enhanced cell-matrix adhesion. In contrast, the antisense-transfection subclone exhibited increased cell-cell aggregation and decreased cell-matrix adhesion. After treatment with sialidase, the cell-matrix adhesion of all the transfectants and the parental MDA-MB-435 cells were significantly reduced to the level of 31%-57% of untreated cells.</p><p><b>CONCLUSION</b>Cell surface sialic acid and alpha2, 6-linked sialylation play an important role in cell-cell and cell-matrix adhesion of mammary carcinoma cell MDA-MB-435.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Sialiltransferasas
/
Neoplasias de la Mama
/
Transfección
/
Antígenos CD
/
Adhesión Celular
/
Membrana Celular
/
Ácido N-Acetilneuramínico
/
Uniones Célula-Matriz
/
Colágeno Tipo IV
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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