Tissue culture and polyploidy induction of Morinda officinalis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2325-2328, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-283202
ABSTRACT
<p><b>OBJECTIVE</b>To establish an effective protocol for plant generation and induce polyploidy of Morinda offcinalis.</p><p><b>METHOD</b>Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry.</p><p><b>RESULT AND CONCLUSION</b>The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg x L(-1) colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Poliploidía
/
Cromosomas de las Plantas
/
Morinda
/
Técnicas de Cultivo de Tejidos
/
Genética
/
Métodos
Tipo de estudio:
Guía de Práctica Clínica
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS