Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2107-2109, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-283247
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.</p><p><b>METHOD</b>An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.</p><p><b>RESULT</b>The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.</p><p><b>CONCLUSION</b>Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Control de Calidad
/
Estándares de Referencia
/
Tortugas
/
Prolina
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Exoesqueleto
/
Glicina
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Artículo
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