Expression of mucosal addressin cell adhesion molecule-1 during small bowel graft rejection in rat / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 421-424, 2006.
Article
en Zh
| WPRIM
| ID: wpr-283305
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of mucosal addressin cell adhesion molecule-1(MAdCAM-1) during small bowel graft rejection and the effects of MAdCAM-1 on the development of acute rejection.</p><p><b>METHODS</b>Rat heterotopic small bowel transplantation (SBT) was performed in F344/N rats with syngeneic and allogeneic (BN-F344/N) grafts. Bowel and gut-associated lymphoid tissue(GALT) samples were collected from small bowel transplants on postoperative day(POD) 1, 3, 5 and 7. Histopathology assessment of the grafts was conducted to identify the rejection. MAdCAM-1 was detected by immunohistochemistry and Western blot.</p><p><b>RESULTS</b>During acute rejection, MAdCAM-1 was highly-expressed on gut lamina propia and GALTs, particularly on vascular endothelial cells in the gut lamina propia. There were no change of MAdCAM-1 expression in syngeneic grafts from POD1 to POD7. In allogeneic grafts, MAdCAM-1 expression in mesenteric lymph nodes was down-regulated, while up-regulated on the vascular endothelial cells in the lamina propria during acute rejection.</p><p><b>CONCLUSION</b>Alteration of MAdCAM-1 expression may be associated with the development of SBT graft rejection.</p>
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Índice:
WPRIM
Asunto principal:
Ratas Endogámicas BN
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Ratas Endogámicas F344
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Trasplante
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Inmunoglobulinas
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Alergia e Inmunología
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Rechazo de Injerto
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Supervivencia de Injerto
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Mucosa Intestinal
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Intestino Delgado
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Ganglios Linfáticos
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2006
Tipo del documento:
Article