Identification of biomarkers in the serum of the patients with poorly differentiated gastric cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 253-256, 2006.
Article
en Zh
| WPRIM
| ID: wpr-283343
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of proteomics in the field of serology,and to screen the differential expression proteins related with poorly differentiated gastric carcinoma.</p><p><b>METHODS</b>Two-dimensional electrophoresis (2-DE) was applied to segregate the total proteins in the serum form gastric cancer patients and health volunteers. After staining,the differential expression proteins were analyzed using PDQuest software,and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>RESULTS</b>Electrophoresis figures with high resolution and reproducibility were obtained. Six differential expression proteins were found only in the serum from gastric cancer patients, while four other proteins from healthy volunteers.</p><p><b>CONCLUSIONS</b>Protein expression is differential in the serum from the gastric cancer patients and health volunteers. It is hopeful to find the biomarkers related with poorly differentiated gastric carcinoma using proteomics.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
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Neoplasias Gástricas
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Sangre
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Electroforesis en Gel Bidimensional
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Biomarcadores de Tumor
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Química
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Proteómica
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Suero
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2006
Tipo del documento:
Article