Studies on preparation of recombinant hirudin-2 liposome and its pharmacokinetics by nasal delivery in rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 801-804, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-283383
ABSTRACT
<p><b>OBJECTIVE</b>To promote the nasal absorption of recombinant hirudin-2, the preparation and physicochemical properties of recombinant hirudin-2 liposomes, as well as its pharmacokinetic characteristics and bioavailability in rats after nasal administration were investigated.</p><p><b>METHOD</b>Recombinant hirudin-2 liposomes were prepared by reversal phase evaporation; the test of physicochemical properties including encapsulation efficiency, particle size and stability of liposome suspensions were determined by HPLC; Recombinant hirudin-2 concentration in plasma was determined by chromogenic substrate method and the relative bioavailability and pharmacokinetic parameters were also calculated using software program 3p87.</p><p><b>RESULT</b>The encapsulation efficiency of recombinant hirudin-2 liposome reached greater than 76.95%, with an average particle size of about 168.3 nm, size distribution ranging from 24 to 286 nm, relative peak width of +/- 0.47, and a good stability.</p><p><b>CONCLUSION</b>Compared with recombinant hirudin-2 solution, liposome preparation enhanced the nasal absorption of recombinant hirudin-2.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tamaño de la Partícula
/
Proteínas Recombinantes
/
Administración Intranasal
/
Portadores de Fármacos
/
Farmacocinética
/
Disponibilidad Biológica
/
Química
/
Hirudinas
/
Tecnología Farmacéutica
/
Ratas Sprague-Dawley
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Artículo
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