Study on residue analysis method of imidacloprid in Ephedrae sinica / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish residue analysis method of imidacloprid in Ephedra sinica by HPLC.</p><p><b>METHOD</b>Imidacloprid was extracted with dichloromethane, cleaned up with chromatography column, then separated on Spherisorb C18 column by using Methanol-water (2080), detected at 270 nm.</p><p><b>RESULT AND CONCLUSION</b>The limit of detection (LOD) and limit quantification (LOQ) were 0.4 x 10(-9) g and 0.02 mg x kg(-1), mean recovery and related standard deviation (RSD) were 85.37%-90.65% and 2.23%-3.45%. It is concluded that the method could satisfy the pesticide residue analysis demands in sensitivity, accuracy and precision.</p>