Determination of butylidenephthalide in Ligusticum chuanxiong by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine butylidenephthalide in Ligusticum Chuanxiong with RP-HPLC.</p><p><b>METHOD</b>The sample was extracted with methanol using sonication. The ESTD was used to quantify butylidenephthalide. HPLC separation was carried out in a Hypersil ODS columm (4.6 mm x 150 mm, 5 microm) , eluted at 1 mL x min(-1) with methanol-5% isopropyl alcohol (60 40) at 25 degrees C. The detection wavelength was 230 nm.</p><p><b>RESULT</b>The linear range was 0.07-0.7 microg for butylidenephthalide. The average recovery was 95.3%, and RSD was 2.3% (n =6).</p><p><b>CONCLUSION</b>This method was simple and could be used to determine butylidenephthalide with satisfactory accuracy and reproducibility.</p>