Antisense hTERT inhibits gene expression and functional activity of telomerase in leukemia cell lines / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 454-457, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-283491
ABSTRACT
<p><b>OBJECTIVE</b>To explore the inhibitory effects of gene expression and functional activity of telomerase in leukemia cell lines by in vitro antisense hTERT treatment.</p><p><b>METHODS</b>An antisense hTERT eukaryotic expression vector was constructed by using gene recombination technique, targeting the 5' end mRNA sequence of the telomerase catalytic subunit. The vector expression in leukemia cell lines (HL60 and K562) was achieved by transfection using the SuperFect transfection reagent (Qiagen). After transfection, ectopic expression of the telomerase catalytic subunit was analyzed by quantitative fluorescence real-time RT-PCR, and cellular apoptosis and cell cycle parameters were evaluated by flow cytometry respectively.</p><p><b>RESULTS</b>An antisense pcDNA-hTERT eukaryotic expression vector was successfully constructed. Leukemia cell lines transfected with antisense hTERT constructed displayed a significant inhibition of gene expression of telomerase and its activity in vitro, as compared with the result of the control groups (without transfection and vector control).</p><p><b>CONCLUSION</b>In-vitro antisense hTERT expression may down-regulate the gene expression and biological activity of telomerase in leukemia cells, suggesting a possibility of gene therapy against human malignancy through the telomerase-targeted molecular mechanism.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes
/
ARN Mensajero
/
Células HeLa
/
Transfección
/
Regulación hacia Abajo
/
Regulación Neoplásica de la Expresión Génica
/
Ciclo Celular
/
ARN sin Sentido
/
Apoptosis
/
Telomerasa
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2004
Tipo del documento:
Artículo
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