Detection of EWS-FLI1 fusion transcript in Ewing's sarcoma/peripheral primitive neuroectodermal tumors by one-step RT-PCR using paraffin-embedded tissues / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 328-331, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-283514
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of EWS-FLI1/ERG fusion transcript resulting from t(11;12)(q24;12) in paraffin-embedded tissues and its diagnostic implication for Ewing's sarcoma/peripheral primitive neuroectodermal tumors (ES/pPNET).</p><p><b>METHODS</b>One-step reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect a characteristic EWS-FLI1/ERG fusion transcript in 25 cases of ES/pPNET and 15 cases of other small round cell tumors (including 8 cases of rhabdomyosarcoma, 4 cases of synovial sarcoma, 2 cases of neuroblastoma and 1 case of lymphoma) using formalin-fixed and paraffin-embedded tissues.</p><p><b>RESULTS</b>EWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%). The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript.</p><p><b>CONCLUSIONS</b>Detection of EWS-FLI1/ERG fusion transcript is a reliable index for molecular diagnosis of ES/pPNET. One-step RT-PCR is a practical method for such analysis in routine paraffin-embedded tumor tissues.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rabdomiosarcoma
/
Sarcoma de Ewing
/
Factores de Transcripción
/
Proteínas de Fusión Oncogénica
/
Adhesión en Parafina
/
Tumores Neuroectodérmicos Periféricos Primitivos
/
Sarcoma Sinovial
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Proteína EWS de Unión a ARN
/
Diagnóstico
Tipo de estudio:
Estudio diagnóstico
Límite:
Adolescente
/
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2004
Tipo del documento:
Artículo
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