The effect of anti-ICAM-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lymphocytes from oral squamous cell carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 107-111, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-283652
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of anti-ICAm-1/LAF-1 McAb on the cytotoxicity of tumor infiltrating lymphocytes from oral squamous cell carcinoma.</p><p><b>METHODS</b>TIL were isolated from fresh tumor tissues in 15 patients with oral squamous cell carcinoma and cultured in vitro. The expression of ICAM-1/LAF-1 in fresh and activated TIL was examined by FACS. The cytotoxicity of TIL against auto tumor cells and the level of TNF-alpha produced by TIL were compared before and after interrupted with anti-ICAM-1/LAF-1 McAb.</p><p><b>RESULTS</b>The expression of ICAM-1 and LAF-1 in the activated TIL significantly increased comparing with fresh TIL (P < 0.05). Interrupted by anti-ICAM-1 McAb, the cytotoxicity of TIL against auto tumor cell was not significantly changed. Interrupted by anti-ICAM-1 and anti-LAF-1 McAb, the level of TNF-alpha produced by TIL showed no differences comparing with the control (P < 0.05), but the cytotoxicity of TIL against auto tumor cells significantly decreased (P < 0.05).</p><p><b>CONCLUSION</b>The effect of anti-LAF-1 McAb may result from steric interference of antigen-nonspecific adhesion process.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias de la Boca
/
Carcinoma de Células Escamosas
/
Células Cultivadas
/
Antígeno-1 Asociado a Función de Linfocito
/
Linfocitos Infiltrantes de Tumor
/
Molécula 1 de Adhesión Intercelular
/
Citotoxicidad Inmunológica
/
Alergia e Inmunología
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2003
Tipo del documento:
Artículo
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