The influence of Ara-C on anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 812-815, 2009.
Article
en Zh
| WPRIM
| ID: wpr-283901
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of Ara-C in regulating anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.</p><p><b>METHODS</b>The diabody of anti-CD3/anti-Pgp was purified by E-tag affinity chromatography. K562 and K562/A02 cells were treated with Ara-C. The expressions of B7-1 and B7-2 on K562 and K562/AO2 cells were detected by FACS. The cytotoxicity of T-lymphocytes combined with anti-CD3/anU-Pgp plus Ara-C was analyzed by CytoTox 96 nonradioactive method.</p><p><b>RESULTS</b>The expressions of B7-1 and B7-2 on K562 and K562/A02 cells treated by Ara-C was significantly higher than those untreated. The effect/target ratio was from 0.39:1 to 25:1, and the killing rate of activated T cells to anti-drug-resistant leukemia cells was from (16.44 +/- 1.20)% to (60.49 +/- 2.90)%. The killing rates were increased gradually, with both the effect/target ratio and the antibody concentration increasing (P < 0.05).</p><p><b>CONCLUSION</b>Ara-C may be an important adjuvant for improving anti-CD3/anti-Pgp mediating T-lymphocytes activities against multi-drug resistant leukemia cells.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Linfocitos T
/
Leucemia
/
Células K562
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Citarabina
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Alergia e Inmunología
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2009
Tipo del documento:
Article