Anti-apoptotic effect of Astragalus Polysaccharide on myeloid cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1243-1247, 2013.
Article
en Zh
| WPRIM
| ID: wpr-283944
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to assess the effect of Astragalus Polysaccharide (ASPS) on in-vitro hematopoiesis. CFU-GM assays were used to determine the effect of ASPS and thrombopoietin (TPO) on granulocytic-monocyte progenitor cells. The CFU assays were also used to investigate the effect of ASPS on the proliferation of HL-60 cells.HL-60 cells were cultured with serum-free RPMI 1640 medium and treated with or without of different concentrations of ASPS. After 72 h incubation, the number of cells were counted.In addition, the caspase-3 and JC-1 expression was determined by flow cytometry with Annexin V/PI double staining. The results showed that ASPS (100, 200 µg/ml) and TPO (100 ng/ml) significantly promoted CFU-GM formation in vitro. Various concentrations of ASPS and TPO also promoted the colony formation of HL-60 cells, the largest effect of ASPS was observed at a concentration of 100 µg/ml. There were no synergistic effects between TPO and ASPS on cellular proliferation. The results also showed that ASPS significantly protected HL-60 cells from apoptosis in condition of serum-free medium culture, suppressed caspase 3 activation, and reduced the cell apoptosis. It is concluded that ASPS can significantly promote the formation of bone marrow CFU-GM and the proliferation of HL-60 cells, the optimal concentration of ASPS is at 100 µg/ml. In the absence of serum inducing apoptosis, ASPS also significantly reduced the apoptosis of HL-60 cells via suppressing the activation of caspase-3.
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Asunto principal:
Farmacología
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Polisacáridos
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Trombopoyetina
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Medicamentos Herbarios Chinos
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Apoptosis
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Células HL-60
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Planta del Astrágalo
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Proliferación Celular
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Caspasa 3
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Hematopoyesis
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2013
Tipo del documento:
Article