Mechanisms of metastasis suppression by introduction of human chromosome 10 into rat prostate cancer / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 123-129, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-284060
ABSTRACT
<p><b>AIM</b>The metastatic ability of a Dunning R-3327 rat prostate cancer subline (AT6.3) was suppressed by the introduction of human chromosome 10, when these hybrid cancer cells were injected subcutaneously into nude mice (Nihei et al., Genes Chromosomes Cancer 14112-119, 1995). The present study was undertaken to clarify which step of metastasis was suppressed in the human chromosome 10-containing microcell hybrids (AT 6.3-10 clones).</p><p><b>METHODS</b>Gelatin zymography, an in vitro invasion assay using a Boyden chamber and an intravenous metastasis assay involving the injection of hybrid cells into nude mice were performed.</p><p><b>RESULTS</b>Gelatin zymography revealed that AT6.3-10 microcell hybrid clones expressed the 72 kD type IV collagenase (MMP-2) at an almost equal level as control microcell hybrid clones. Both the invasiveness as measured by the invasion assay and the number of lung metastases as measured by the intravenous metastasis assay of AT6.3-10 hybrid clones were significantly less than those of the AT6.3 parental clone.</p><p><b>CONCLUSION</b>The human chromosome 10 suppresses both the local invasion and the metastatic process after entry into the blood circulation of rat prostate cancer. This decrease in local-invasive ability does not seem to require a decrease in MMP-2 activity.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias de la Próstata
/
Neoplasias Cutáneas
/
Cromosomas Humanos Par 10
/
Animales Modificados Genéticamente
/
Células Tumorales Cultivadas
/
División Celular
/
Gelatina
/
Genética
/
Ratones Endogámicos BALB C
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Asian Journal of Andrology
Año:
2002
Tipo del documento:
Artículo
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