Identification of occult disseminated tumor cells by recombinant herpes simplex virus expressing GFP (HSV(GFP)) / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 894-898, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-284262
ABSTRACT
<p><b>OBJECTIVE</b>To develop a novel rapid protocol for the detection of occult disseminated tumor cells by a recombinant herpes simplex virus expressing GFP (HSV(GFP)).</p><p><b>METHODS</b>Tumor cells of seven cell lines were exposed to HSV(GFP) and then examined for GFP expression by fluorescence microscopy. Various numbers of tumor cells (10, 100, 1000, 10 000) were mixed into 2 ml human whole blood, separated with lymphocytes separation medium, exposed to HSV(GFP), incubated at 37°C for 6 - 24 h and then counted for the number of green cells under the fluorescence microscope. Some clinical samples including peripheral blood, pleural effusion, ascites, spinal fluid from tumor-bearing patients were screened using this protocol in parallel with routine cytological examination.</p><p><b>RESULTS</b>HSV(GFP) was able to infect all 7 tumor cell lines indicating that the HSV(GFP) can be used to detect different types of tumor cells. The detection sensitivity was 10 cancer cells in 2 ml whole blood. In the clinical samples, there were 4/15 positive by routine cytological examination but 11/15 positive by HSV(GFP), indicating a higher sensitivity of this new protocol.</p><p><b>CONCLUSION</b>Recombinant herpes simplex virus-mediated green fluorescence is a simple and sensitive technique for the identification of occult disseminated cancer cells including circulating tumor cells (CTCs).</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Células Vero
/
Proteínas Recombinantes
/
Chlorocebus aethiops
/
Sensibilidad y Especificidad
/
Simplexvirus
/
Línea Celular Tumoral
/
Proteínas Fluorescentes Verdes
/
Metabolismo
/
Células Neoplásicas Circulantes
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2012
Tipo del documento:
Artículo
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