Establishment of a new method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 343-347, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-284372
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new culture method to induce the differentiation of embryonic pancreatic cells into mature endocrine cells.</p><p><b>METHODS</b>Mouse embryos at day 12.5 were used and embryonic pancreata were isolated. The isolated embryonic pancreata were cultured on the filter for 7 days, which floated in the dish containing medium. During culture, the expression of pancreas duodenum homeobox-1 (PDX-1), a pancreatic stem cell marker, was examined at day 1. The expression of neurogenin 3 (Ngn3), a pancreatic progenitor cell marker, was examined at day 3. The expressions of endocrine and exocrine markers, insulin, glucagon, and carboxypeptidase (CPA) were examined at day 7 by immunohistochemistry. The kinetics of pancreatic marker expression during culture was assayed by real-time PCR.</p><p><b>RESULTS</b>Many pancreatic stem cells still existed in embryonic pancreata cultured for 1 day; meanwhile, these pancreatic stem cells proliferated in high rate. A large amount of pancreatic progenitor cells were found in embryonic pancreata cultured for 3 days.Pancreatic stem/progenitor cells differentiated into mature endocrine and exocrine cells in embryonic pancreata after having been cultured for 7 days. Furthermore, the expression pattern of pancreatic marker is consistent with that in vivo.</p><p><b>CONCLUSION</b>We successfully established a new culture method, with which embryonic pancreatic cells can efficiently differentiate into mature endocrine cell.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Páncreas
/
Diferenciación Celular
/
Transactivadores
/
Células Cultivadas
/
Proteínas de Homeodominio
/
Técnicas de Cultivo de Célula
/
Biología Celular
/
Embrión de Mamíferos
/
Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
/
Células Endocrinas
Límite:
Animales
Idioma:
Inglés
Revista:
Acta Academiae Medicinae Sinicae
Año:
2012
Tipo del documento:
Artículo
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