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Expression of pax-6 in rhesus monkey of optical defocus induced myopia and form deprivation myopia / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 722-726, 2004.
Artículo en Inglés | WPRIM | ID: wpr-284925
ABSTRACT
<p><b>BACKGROUND</b>Pax-6 gene plays an important role in the process of eye development. This study was to determine the role of pax-6 in the axial myopia produced by hyperopic optical defocus and form deprivation in infant monkeys.</p><p><b>METHODS</b>Among seven normal infant rhesus monkeys (aged 1 to 1.5 months), five wore -3.00 D spectacle lenses over their right eyes and zero-powered lenses over their left eyes. Monocular form deprivation was produced by eyelid fusion in two monkeys. Ten weeks later, the monkeys were sacrificed by an overdose of barbiturates and their eyes were removed immediately. A 5 mm x 5 mm button of retina and sclera was taken from the posterior poles along with a 4-mm optic nerve. RNA was isolated separately from each of these three types of tissues. After that, reverse transcription polymerase chain reaction (RT-PCR) was used for determining gene expression in the retina, sclera and optic nerve. Semi-quantitative analyses were performed on the PCR products.</p><p><b>RESULTS</b>As expected, the optically induced hyperopic defocus and the form deprivation produced myopic growth. For the lens-treatment monkeys, pax-6 gene expression in the retinas of the defocused eyes was significantly higher than in the retinas of the left eyes (t = 5.703, P = 0.005). However, there were no analogous significant differences between pax-6 expression in the scleras or the optic nerves. For the two form-deprived monkeys, there were no obvious differences in pax-6 gene expression in the retinas or the optic nerves.</p><p><b>CONCLUSION</b>The result that the expression of pax-6 was enhanced by hyperopic defocus in the infant monkey retina suggests that pax-6 may be involved in vision-dependent eye growth and emmetropization.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Nervio Óptico / Patología / Proteínas Represoras / Retina / Esclerótica / Regulación de la Expresión Génica / Proteínas de Homeodominio / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Factores de Transcripción Paired Box / Proteínas del Ojo Límite: Animales Idioma: Inglés Revista: Chinese Medical Journal Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Nervio Óptico / Patología / Proteínas Represoras / Retina / Esclerótica / Regulación de la Expresión Génica / Proteínas de Homeodominio / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Factores de Transcripción Paired Box / Proteínas del Ojo Límite: Animales Idioma: Inglés Revista: Chinese Medical Journal Año: 2004 Tipo del documento: Artículo