The detection of mutations in VHL gene from a single cell in a patient with von Hippel-Lindau disease / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 67-71, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-285031
ABSTRACT
<p><b>OBJECTIVE</b>To explore a technology for diagnosing VHL mutations from a single cell and provide experimental evidences for the feasibility of applying technology in detecting genetic mutations from a single cell.</p><p><b>METHODS</b>After whole genome amplification (WGA) based on multiple displacement amplication (MDA) for a single cell, we did regular PCR following sequencing and detected the genotypes using the real time PCR based on TaqMan probes. We detected VHL mutations by the different terminal fluorescent changing.</p><p><b>RESULTS</b>The rate of amplification for single cell based on MDA was 90.91%. The rate of contamination was 0. After sequencing, the allele drop out (ADO) rate of heterozygotes was 26.67%(8/30); combined with the different terminal fluorescent changing, the rate of ADO of heterozygotes was 16.67%.</p><p><b>CONCLUSION</b>WGA based on MDA for a single cell followed by regular PCR with sequencing and real time PCR can specially and accurately detect the VHL genotypes of single cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Sangre
/
Análisis Mutacional de ADN
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Linfocitos
/
Reacción en Cadena de la Polimerasa
/
Diagnóstico Preimplantación
/
Proteína Supresora de Tumores del Síndrome de Von Hippel-Lindau
/
Genética
Tipo de estudio:
Estudio diagnóstico
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2007
Tipo del documento:
Artículo
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