Methylation status of FHIT gene in plasma and expression of FHIT gene in cancer tissue of cervical cancer patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 565-567, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-285075
ABSTRACT
<p><b>OBJECTIVE</b>To explore the methylation status of 5' CpG island of fragile histidine triad (FHIT) gene in plasma and the expression of FHIT protein in cancer tissue of cervical cancer patients.</p><p><b>METHODS</b>Methylation-specific PCR (MS-PCR) was employed to examine methylation of FHIT gene in 151 plasma samples before treatment. The immunohistochemistry was used to the expression of FHIT protein in cancer tissues.</p><p><b>RESULTS</b>CpG island methylation of FHIT was detected in 31.13% of plasma samples. The expression of FHIT protein was decreased or discarded in 59.60% of cervical cancer tissues. Among them 47.78% was included in methylation positive samples.</p><p><b>CONCLUSION</b>CpG island methylation of FHIT gene in plasma plays an important role on cervical cancer, which results in decreased expression of FHIT protein. It can be used to diagnose and evaluate the effect of treatment to cervical cancers.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Inmunohistoquímica
/
Neoplasias del Cuello Uterino
/
Reacción en Cadena de la Polimerasa
/
Ácido Anhídrido Hidrolasas
/
Metilación de ADN
/
Genética
/
Metabolismo
/
Proteínas de Neoplasias
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2006
Tipo del documento:
Artículo
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