Determination of quercetin-3-O-alpha-L-arabinopyranoside in Periploca forrestii by RP-HPLC / 中国中药杂志

ABSTRACT

The aim of the paper was to develop a HPLC method for the quality control of Periploca forrestii Schltr. The 18 samples were analyzed on a Hypersil C18 column. The mobile phase was methanol-water (3367) and the flow rate was 1 mL x min(-1). The detection wavelength was at 370 nm and column temperature was 25 degrees C. The linear relationship was good (r = 0.999 9) in the range of 0.204 4-2.044 microg for quercetin-3-O-alpha-L-arabinopyranoside. The average recovery was 97.78% (RSD 0.8%, n = 9). The contents of 18 samples varied from 0.171% to 0.264%. The method showed high precision, good repeatability and stability, so it can be used to assess the quality of P. forrestii.