Inhibition of panaxynol on experimental delayed type hypersensitivity / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect of panaxynol (PAN) on delayed type hypersensitivity and possible mechanism.</p><p><b>METHOD</b>Allergic contact dermatitis (ACD) was induced by DNCB as a delayed type hypersensitivity (DTH) model to observe effect of PAN on auricle inflammation including pathological injury. Proliferation of T lymphocytes was induced by ConA and measured by MTf method. IFN-gamma secretion of splenocyte induced by ConA was detected by ELISA.</p><p><b>RESULT</b>The swelling degree of auricle and pathological injury in ACD mice was reduced significantly by treated with PAN in induction phase. Proliferation of T lymphocytes induced by ConA in vitro was inhibited significantly by PAN, By contrast, no detectable effect was observed in resting splenocyte. IFN-y induced by ConA in splenocytes was inhibited markedly by PAN from 10 micromol x L(-1) and from 6 h.</p><p><b>CONCLUSION</b>The results showed that DTH was inhibited by PAN mainly in induction phase and this effect may be related with the inhibition on T lymphocytes proliferation and secretion of IFN-gamma.</p>