Identification of proteins interacted with Bat3 using tandem affinity purification / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 1-4, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-285935
ABSTRACT
<p><b>OBJECTIVE</b>To identify the specific protein interactions involved in Bat3-mediated apoptosis.</p><p><b>METHODS</b>Tandem affinity purification (TAP) was utilized to investigate Bat3-protein interactions, during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions. The isolated proteins were identified with mass spectrometry.</p><p><b>RESULTS</b>TAP studies showed that Ubl4A was identified as a Bat3-binding partner. Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.</p><p><b>CONCLUSION</b>TAP was successfully established and is suitable for isolating the binding partners of Bat3.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Unión Proteica
/
Ubiquitinas
/
Línea Celular
/
Chaperonas Moleculares
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2014
Tipo del documento:
Artículo
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