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Effects of Jinmaitong capsule on oxidative stress and cell apoptosis of dorsal root ganglion in diabetic rats / 中国医学科学院学报
Zhongguo yi xue ke xue yuan xue bao ; Zhongguo yi xue ke xue yuan xue bao;(6): 649-654, 2013.
Article en Zh | WPRIM | ID: wpr-285944
Biblioteca responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Jinmaitong capsule on oxidative stress and cell apoptosis of dorsal root ganglion (DRG) in rats with diabetic peripheral neuropathy.</p><p><b>METHODS</b>Sixty male SD rats were randomly divided into normal group and model groups. The diabetic rat models were established using Streptozotocin (STZ) method (60 mg/kg of intraperitoneal injection), and then randomly divided Jinmaitong low, middle, and high-dose groups and vitamin C group. All the experimental rats were sacrificed at 16-week and then the DRG was isolated. The morphological changes of DRG were observed using the Nissl's staining, and the NADPH oxidase subunit p22-phox, Cyt C, Bcl-2, and Caspase-3 of DRG in rats were detected by immunohistochemistry and quantitative reverse transcription PCR (qRT-PCR). Cell apoptosis was detected by TUNEL.</p><p><b>RESULTS</b>Compared with the model group, the expressions of NADPH oxidase subunit p22-phox protein, Cyt expression of C protein, Caspase-3 protein, and mRNA cell apoptosis rate in each treatment group significantly decreased whereas the expressions of Bcl-2 mRNA and protein significantly increased (P<0.05 or P<0.01). The Jinmaitong high-dose group had the best effect and was significantly different from that of the vitamin C group (P<0.01).</p><p><b>CONCLUSIONS</b>Jinmaitong capsule can prevent the nerve injury in rats with diabetic peripheral neuropathy by inhibiting oxidative stress and decreasing the apoptosis. The high-dose Jinmaitong capsule has the best effect and is superior to vitamin C.</p>
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Farmacología / ARN Mensajero / Cápsulas / Medicamentos Herbarios Chinos / Ratas Sprague-Dawley / Apoptosis / Estrés Oxidativo / Usos Terapéuticos / Diabetes Mellitus Experimental / Neuropatías Diabéticas Límite: Animals Idioma: Zh Revista: Zhongguo yi xue ke xue yuan xue bao Año: 2013 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Farmacología / ARN Mensajero / Cápsulas / Medicamentos Herbarios Chinos / Ratas Sprague-Dawley / Apoptosis / Estrés Oxidativo / Usos Terapéuticos / Diabetes Mellitus Experimental / Neuropatías Diabéticas Límite: Animals Idioma: Zh Revista: Zhongguo yi xue ke xue yuan xue bao Año: 2013 Tipo del documento: Article