Fusion of melanoma cells using a modified phytohaemagglutinin-ECM830 fusion method / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 515-518, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-285968
ABSTRACT
<p><b>OBJECTIVE</b>To study melanoma cell fusion and find a highly efficient fusion method for tumor cells.</p><p><b>METHODS</b>Melanoma cells were labeled with green fluorescent protein and red fluorescent protein, respectively, and fused by a modified phytohaemagglutinin (PHA)-ECM830 fusion method. Melanoma fusion cells were selected by the fluorescence activated cell sorting. DNA content was determined by propidium iodide staining.</p><p><b>RESULTS</b>Melanoma cells were labeled with green fluorescent protein and red fluorescent protein markers and successfully fused through PHA-ECM830 fusion method. The fusion efficiency (7.18%) was much higher compared with ECM830 electricfusion method (0.50%). Melanoma fusion cells were successfully obtained by the fluorescence activated cell sorting.DNA content was doubled in melanoma fusion cells compared to B16-F10 melanoma cells. The proliferation rate of melanoma fusion cells was significantly decreased in vitro and in vivo.</p><p><b>CONCLUSIONS</b>We successfully obtained the melanoma fusion cells by the improved PHA-ECM830 fusion method. The proliferation rate of melanoma fusion cells dramatically decreases.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Fitohemaglutininas
/
Melanoma Experimental
/
Fusión Celular
/
Línea Celular Tumoral
/
Proliferación Celular
/
Métodos
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2013
Tipo del documento:
Artículo
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