Expression and characterization of rabies virus nucleoprotein in baculovirus / 病毒学报
Chinese Journal of Virology
;
(6): 351-356, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-286113
ABSTRACT
To construct a recombinant expression plasmid Bacmid-N containing the N gene of Rabies Virus, the N gene of RV CVS-11 strain was cloned into the baculovirus shuttle vector (Bacmid). Recombinant Baculovirus AcMNPV-N (P1 Viral stock) was obtained by transfecting the Bacmid-N into the insect cell line of Sf9. The expressed nucleoprotein was identified and analysised by ELISA, FA, SDS-PAGE and Western blot assays. The results showed that the NP protein was expressed intracellularly and had a good antigenicity, which would be potentially used for further study on the diagnostic reagent of rabies virus detection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virus de la Rabia
/
ADN Viral
/
Ensayo de Inmunoadsorción Enzimática
/
Línea Celular
/
Baculoviridae
/
Spodoptera
/
Técnica del Anticuerpo Fluorescente Indirecta
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Genética
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2010
Tipo del documento:
Artículo
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