Your browser doesn't support javascript.
loading
Cloning, prokaryotic expression of chicken interferon-alpha gene and study on antiviral effect of recombinant chicken interferon-alpha / 生物工程学报
Chinese Journal of Biotechnology ; (12): 737-743, 2006.
Artículo en Chino | WPRIM | ID: wpr-286217
ABSTRACT
The full length of chicken interferon alpha (ChIFN-alpha) gene was amplified by the polymerase chain reaction (PCR) from total liver genome of Sanhuang meat-chicken and sequenced. The amplified gene was about 582bp. The coding region for mature protein (489bp) was subcloned into pET-28a(+). The recombinant plasmid pET-28a(+)-IFNalpha was identified by enzyme digestion and DNA sequencing. Data of SDS-PAGE and Western-blot indicated that a 22kD fusion protein was expressed in the form of inclusion bodies with good immunity. The purity of inclusion bodies was above 70% and that of protein purified by nickel affinity chromatography was 95%. The recombinant protein could inhibit H9N2 avian influenza virus (H9N2 AIV) replication on chick embryo fibroblast. 2 microg of recombinant IFN-alpha could completely protect Chick embryo from H9N2 AIV infection. The recombinant IFN-alpha can also delay Newcastle disease virus (NDV) replication on chick embryo for 12 approximately 48h. Chicken administered recombinant IFN-alpha can resist the H9N2 AIV infection. The bioactivities of recombinant IFN-alpha purified by affinity chromatograph were 20 times higher than that of inclusion bodies.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Antivirales / Farmacología / Plásmidos / Proteínas Recombinantes / Virus de la Enfermedad de Newcastle / Interferón Tipo I / Western Blotting / Interferón-alfa / Clonación Molecular / Subtipo H9N2 del Virus de la Influenza A Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2006 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Antivirales / Farmacología / Plásmidos / Proteínas Recombinantes / Virus de la Enfermedad de Newcastle / Interferón Tipo I / Western Blotting / Interferón-alfa / Clonación Molecular / Subtipo H9N2 del Virus de la Influenza A Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2006 Tipo del documento: Artículo