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Purification and properties of isoflavone-glucosidase / 生物工程学报
Chinese Journal of Biotechnology ; (12): 635-638, 2006.
Artículo en Chino | WPRIM | ID: wpr-286236
ABSTRACT
A high activity isoflavone-glucosidase, which hydrolysis glycosides, was obtainde using liquid fermentation from Absidia sp. R strain. The isoflavone-glucosidase was purified 11 folds with yielding rate of 10.9% after ammonium sulfate precipitation and DEAE-Cellocuse (DE-52) ion exchange chromatography. SDS-PAGE results showed that the molecular weight is 53kD. And the optimum temperature, the optimum pH, Km and pI of the enzyme are 50 deegrees C, 5.0, 1.3 x 10(-2) mol/L and 3.2, respectively. The isoflavone-glucosidase is also rather stable under 60 degrees C and in pH range from 5.0 to 7.0. The enzyme can be activated by Co2+ and Ca2+, and be inhibited by Ag+ and Cu2+.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Temperatura / Absidia / Glucosidasas / Concentración de Iones de Hidrógeno / Isoflavonas / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Temperatura / Absidia / Glucosidasas / Concentración de Iones de Hidrógeno / Isoflavonas / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2006 Tipo del documento: Artículo