Study of fusion protein and attachment glycoprotein of Nipah virus expressed in recombinant baculovirus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 418-424, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-286273
ABSTRACT
In this study, Recombinant baculoviruses rBac-NF and rBac-NG were generated for expressing F and G proteins Nipah virus (NiV) . The expression of recommbinant G (rNG) and F (rNF) protein in rBac-NF and rBac-NG infected cells were confirmed by western-blot. Both rNG and rNF showed sensitive and specific antigenic reaction to rabbit serum anti-Nipah virus in indirect immunofluorescence detection and indirect ELISA. Immunization with rBac-NF and rBac-NG infected insect cells elicited G ad F protein specific antibody responses in mice. Furthermore, the G ad F specific antibodies could neutralize the infectivity of the VSVdeltaG* F/G, the NiV F and G envelope glycoproteins psudotyped recombinant Vesicular Stomatitis Virus expressing green fluorescence protein. The results demonstrated F and G protein expressed by the recombinant baculoviruses could be safe economic diagnostic antigens for the surveillance and monitoring of NiV and promising subunit vaccines for the prevention of NiV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Recombinación Genética
/
Proteínas Recombinantes de Fusión
/
Química
/
Proteínas del Envoltorio Viral
/
Baculoviridae
/
Virus Nipah
/
Alergia e Inmunología
/
Genética
/
Metabolismo
/
Ratones Endogámicos BALB C
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2006
Tipo del documento:
Artículo
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