Expression of human LNalpha4LG4-5 in Pichia yeast / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 226-230, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-286304
ABSTRACT
To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pichia
/
Plásmidos
/
Proteínas Recombinantes de Fusión
/
Línea Celular
/
Laminina
/
Vectores Genéticos
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2006
Tipo del documento:
Artículo
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