Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 982-986, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-286613
ABSTRACT
After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four generation passage, the virus harvested from sucking mice was confirmed to be type O FMDV by the indirect hemagglutination test, sucking mice's neutralization test and sequencing. The results showed that we have successfully constructed the full-length cDNA clone of FMDV O/QYYS/s/06 strain.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transcripción Genética
/
Virología
/
Virulencia
/
ADN Viral
/
Transfección
/
Clasificación
/
Clonación Molecular
/
ADN Complementario
/
Virus de la Fiebre Aftosa
/
Fiebre Aftosa
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Artículo
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