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A prime-boost vaccination strategy using a Semliki Forest virus replicon vectored DNA vaccine followed by a recombinant adenovirus protects pigs from classical swine fever / 生物工程学报
Chinese Journal of Biotechnology ; (12): 679-685, 2009.
Artículo en Chino | WPRIM | ID: wpr-286657
ABSTRACT
We have previously evaluated a Semliki Forest virus (SFV) replicon vectored DNA vaccine (pSFV1CS2-E2) and a recombinant adenovirus (rAdV-E2) expressing the E2 glycoprotein of classical swine fever virus (CSFV) in pigs. The results showed that the immunized pigs were protected from virulent challenge, but few pigs showed short-term fever and occasional pathological changes following virulent challenge. To enhance the immunogenecity of the vaccines, we tried a prime-boost vaccination strategy using a combination of prime with pSFV1CS2-E2 followed by boost with rAdV-E2. The results showed that all the immunized pigs developed high-level CSFV-specific antibodies following prime-boost immunization. When challenged with virulent CSFV, the immunized pigs (n = 5) from the heterologous boost group showed no clinical symptoms, and CSFV RNA was not detected following challenge, whereas one of five pigs from the homologous boost group developed short-term fever and CSFV RNA was detected. This demonstrates that the heterologous prime-boost vaccination regime has the potential to prevent against virulent challenge.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Replicón / Virus de los Bosques Semliki / Porcinos / Vacunas Virales / Adenoviridae / Proteínas del Envoltorio Viral / Inmunización Secundaria / Proteínas E2 de Adenovirus / Vacunas de ADN / Alergia e Inmunología Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Replicón / Virus de los Bosques Semliki / Porcinos / Vacunas Virales / Adenoviridae / Proteínas del Envoltorio Viral / Inmunización Secundaria / Proteínas E2 de Adenovirus / Vacunas de ADN / Alergia e Inmunología Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2009 Tipo del documento: Artículo