Genetic improvement of alpha-amylase producing Bacillus licheniformis by homolog-mediated alpha-amylase gene amplification / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 375-380, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-286702
ABSTRACT
Bacillus licheniformis alpha-amylase (BLA) is one of the most important enzymes involved in starch hydrolysis and many biotechnological processes. To improve the BLA productivity, an integrative plasmid pBL-amyL carrying amyL gene encoding a thermophilic alpha-amylase of B. licheniformis was constructed and transformed into B. licheniformis B0204, an industrial alpha-amylase producer. The transformants harboring different copies of amyL were developed on kanamycin by using homolog-mediated chromosomal amplification of alpha-amylase gene. The recombinants with different multiple copies of amyL integrated in the chromosome were identified by real-time PCR and evaluated by shake-flask fermentation. Recombinants harboring 2-5 multiple copies of amyL produced more alpha-amylase comparison to the parental strain B0204.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bacillus
/
Transformación Genética
/
Microbiología Industrial
/
Amplificación de Genes
/
Técnicas de Amplificación de Ácido Nucleico
/
Alfa-Amilasas
/
Genética
Idioma:
Inglés
Revista:
Chinese Journal of Biotechnology
Año:
2009
Tipo del documento:
Artículo
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