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Arctiin ameliorates advanced oxidation protein product-induced epithelial-to- mesenchymal transition in HK-2 cells by inhibiting endoplasmic reticulum stress / 南方医科大学学报
Journal of Southern Medical University ; (12): 833-837, 2016.
Artículo en Chino | WPRIM | ID: wpr-286889
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of arctiin on advanced oxidation protein product (AOPP)-induced epithelial-to-mesenchymal transition (EMT) in tubular cells and explore the mechanisms underlying this effect.</p><p><b>METHODS</b>Human proximal tubular cells (HK-2 cells) were treated with bovine serum albumin (BSA) or AOPPs in the presence or absence of arctiin. The expressions of E-cadherin, vimentin, and GRP78 at the protein and mRNA levels in the cells were examined using Western blotting and quantitative real-time PCR. The level of reactive oxygen species (ROS) was measured by flow cytometry with DCFH-DA as the fluorescent probe.</p><p><b>RESULTS</b>Compared with BSA-treated cells, the cells treated with AOPPs showed decreased expression of epithelial cell marker E-cadherin and overexpression of mesenchymal marker vimentin and endoplasmic reticulum stress marker GRP78 with an increased ROS level. These changes induced by AOPPs were partly inhibited by arctiin.</p><p><b>CONCLUSION</b>Arctiin can ameliorate AOPP-induced EMT in tubular cells by inhibiting endoplasmic reticulum stress, and oxidative stress response may participate in this process.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Vimentina / Cadherinas / Línea Celular / Especies Reactivas de Oxígeno / Estrés Oxidativo / Biología Celular / Células Epiteliales / Transición Epitelial-Mesenquimal / Estrés del Retículo Endoplásmico Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Vimentina / Cadherinas / Línea Celular / Especies Reactivas de Oxígeno / Estrés Oxidativo / Biología Celular / Células Epiteliales / Transición Epitelial-Mesenquimal / Estrés del Retículo Endoplásmico Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2016 Tipo del documento: Artículo