A cell model of ischemia/reperfusion injury on skeletal muscle / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To establish a model of ischemia/reperfusion injury on L-6TG cell.</p><p><b>METHODS</b>Cultured L-6TG cells were divided into 2 groups control group (C), ischemia/reperfusion group (I/R), LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochondria respiration were evaluated in each group, the micromorphologic changes were observed with microscope.</p><p><b>RESULTS</b>Compared with control group, after L-6TG cell suffered ischemia 4 hours and reperfusion 4 hours, LDH in culture fluid, XOD, free calcium in L-6TG cell all increased significantly, while SOD in L-6TG cell and mitochondrial respiration decreased, structural damage to L-6TG cell was severe.</p><p><b>CONCLUSION</b>Using mimicking ischemic solution and mimicking reperfusion solution can successfully establish a model of ischemia/reperfusion injury on L-6TG cell.</p>