Detection of distinct proteins for brain injury rats serum using a protein chip / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 270-273, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-287037
ABSTRACT
<p><b>AIM</b>To explore the alteration of serum protein fingerprinting of rats subjected brain injury.</p><p><b>METHODS</b>Male Sprague-Dawley rats were randomly divided into seven groups, ie, normal control group, sham operation group, injury 4 h, 8 h, 12 h, 24 h and 48 h groups. The change of protein pattern in serum was monitored with weak cationic exchanger chip and surface-enhanced laser desorption ionization-time of flight-mass spectrometry.</p><p><b>RESULTS</b>The reduction of 5648 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.01, compared with control and sham operation) and recovery at 24 h or 48 h. The increase of 9681 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.05, compared with control and sham operation) and recovery at 24 h or 48 h.</p><p><b>CONCLUSION</b>The alteration of protein expression in serum could be induced after brain injury.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Lesiones Encefálicas
/
Proteínas Sanguíneas
/
Ratas Sprague-Dawley
/
Análisis por Matrices de Proteínas
/
Suero
/
Modelos Animales de Enfermedad
/
Metabolismo
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2005
Tipo del documento:
Artículo
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