A fluorospectrophotometric determination of nitrite in blood / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 235-239, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-287048
ABSTRACT
<p><b>AIM</b>To establish a fluorospectrophotometric assay for the measurement of nitrite in blood.</p><p><b>METHODS</b>Interference from hemoglobin and other blood ingredients was removed through sulfuric acid and phosphotungstic acid pretreatment. Fluorescence of 1-[H]-naphthotriazole from the reaction of 2,3-diaminonaphthalene with nitrite was determined with fluorospectrophotometry.</p><p><b>RESULTS</b>The following conditions were proper Serum or plasma was treated with sulfuric acid and phosphotungstic acid pretreatment for two times, 2,3-diaminonaphthalene of 0.63 mmol x (L(-1)) was used, reaction solution pH and final pH were about 1.60 and 1.70 respectively, solution containing 2,3-diaminonaphthalene and supernatant after pretreatment was water-bathed at 20 degrees C for 15 minutes. The lower limit of detection was 24.27 nmol x L(-1). Nitrite determined in peripheral blood of healthy people was (10.91 +/- 2.38) micromol x L(-1), and its 95% distribution range was (6.24-15.57) micromol x L(-1).</p><p><b>CONCLUSION</b>It's a relatively sensitive, specific, simple method. It's of some value to the study of nitric oxide.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Fluorofotometría
/
Límite de Detección
/
Nitritos
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2005
Tipo del documento:
Artículo
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