Proteomics analysis on stressed myocardium injury-related proteins / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 171-174, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-287066
ABSTRACT
<p><b>AIM</b>To probe the related proteins to stress-induced myocardium injury.</p><p><b>METHODS</b>After establishment of a myocardium injury model induced by restraint stress in rats, myocardium proteins of restraint stress-treated and untreated rats were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) and SDS-PAGE two-dimensional electrophoresis respectively. The alterative protein spots were analyzed by Image Master 3.01 software and identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.</p><p><b>RESULTS</b>Proteomics analysis showed that there were 10 proteins were significantly influenced by restraint stress in rat myocardium. After stress, proteins, including cardiac myosin heavy chain, dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, similar to dihydrolipoamide S-succinyltransferase, mitochondrial aldehyde dehydrogenase, H (+)-transporting ATP synthase, albumin, myosin heavy chain and apolipoprotein A-I precursor showed increased expression. Mitochondrial aconitase and uncoupling protein UCP-3 showed decreased expression.</p><p><b>CONCLUSION</b>These differential expressive proteins might be involved in stress-induced injury to myocardium.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estrés Fisiológico
/
Restricción Física
/
Ratas Wistar
/
Proteómica
/
Modelos Animales de Enfermedad
/
Proteínas de Choque Térmico
/
Metabolismo
/
Miocardio
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2005
Tipo del documento:
Artículo
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