Isolation and identification of spermatids from mouse testis / 中华男科学杂志
National Journal of Andrology
;
(12): 28-31, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-287212
ABSTRACT
<p><b>OBJECTIVES</b>To develop a simple and effective method by which spermatids can be isolated from mouse testis.</p><p><b>METHODS</b>Combination of enzymatic digestion was used to prepare suspension of spermatogenic cells from adult mouse testis, and then a modified discontinuous Percoll gradient (15%, 22%, 30%, 40%, 50%, 60%) centrifugation method was introduced to isolate spermatids from the cellular suspension. The content of spermatids in each isolated fraction by Percoll method was determined by morphology (Wright-Giemsa staining) and flow cytometry analysis, and the viability of spermatogenic cells was assessed using Eosin Y exclusion test.</p><p><b>RESULTS</b>More than 97% of the testicular cells remained their viability after enzymatic digestion. After Percoll centrifuged, six fractions were formed. In each isolated fraction, the 22% fraction contained mostly spermatids(mean 86.7%) and cell viability was more than 85.5%. While in the 30% fraction, immature spermatogenic cells were present, and more than 92% of the cells remained their viability.</p><p><b>CONCLUSIONS</b>A large of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined discontinuous Percoll gradient centrifugation method.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espermátides
/
Testículo
/
Separación Celular
/
Centrifugación por Gradiente de Densidad
/
Biología Celular
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2002
Tipo del documento:
Artículo
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