Protect effects of Qingkailing injection on mitochondrion membrane potential during injury induced by hypoxia-hypoglycemia and reoxygenation in cultured rat hippocampal neurons / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1852-1855, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-287271
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protect effects of Qingkailing injection on mitochondrion membrane potential (MMP) during injury induced by hypoxia-hypoglycemia and reoxygenation in cultured rat hippocampal neurons.</p><p><b>METHOD</b>Mitochondrion activity was measured by methyl thiazolyl tetrazolium (MTT) test. MMP and apoptosis were measured by flow cytometry. intracellular free calcium concentration ([Ca2+]i) was measured with confocal laser scanning microscopy.</p><p><b>RESULT</b>Hypoxia-hypoglycemia cultures for 5 hour and reoxygenation for 3 hour induced intracellular[Ca2+]i and apoptosis rate significantly increased. The effects were increased with the extending time of reoxygenation. MMP and mitochondrion activity declined significantly after 3 hour reoxygenation. The effects were declined with the extending time of reoxygenation. Qingkailing injection might have significantly decrease intracellular [Ca2+]i and apoptosis rate, increase MMP and mitochondrion activity.</p><p><b>CONCLUSION</b>Qingkailing Injection might have significantly inhibit the decline in MMP induced by hypoxia-hypoglycemia and reoxygenation, and had effects of stable it and anti-neuronal apoptosis. The effects might be related to inhibit overload of intracellular free calcium.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Fisiología
/
Plantas Medicinales
/
Medicamentos Herbarios Chinos
/
Distribución Aleatoria
/
Hipoxia de la Célula
/
Células Cultivadas
/
Química
/
Calcio
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2005
Tipo del documento:
Artículo
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