Curcumin combined FOLFOX induced cell apoptosis of gastric cancer and its mechanism research / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 810-813, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-287463
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of curcumin combined folinic acid fluorouracil oxaliplatin (FOLFOX) on the gastric adenocarcinoma cell line BGC-823 and to explore its possible mechanisms.</p><p><b>METHODS</b>Cells were divided into five groups, i.e. the blank control group, the curcumin group, the FOLFOX group (0.1 mmol/L 5-FU +5 micromol/L oxaliplatin), and the curcumin combined FOLFOX group. CCK-8 was used to detect cell activity. The cell apoptosis was observed using Hoechst dyeing. Caspase-3 test kit was applied to test Caspase-3 vitality. The mRNA expressions of Bcl-2 and Bax were detected by real time fluorescent quantitative PCR. The expressions of Bcl-2 and Bax protein were determined by Western blot.</p><p><b>RESULTS</b>The BGC-823 cells' proliferation could be inhibited, apoptosis induced, the Caspase-3 activity increased, expressions of Bcl-2 mRNA and Bcl-2 protein lowered, while Bax mRNA and Bax protein expressions increased in each medicated group. Besides, the efficacy of the curcumin combined FOLFOX group was superior to that of the curcumin group and the FOLFOX group, showing statistical difference (P < 0.01).</p><p><b>CONCLUSION</b>Curcumin combined FOLFOX could significantly inhibit the proliferation of BGC-823 cells possibly via promoting Bax expression and Caspase-3 activity, inhibiting Bcl-2 expression, thus inducing apoptosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Compuestos Organoplatinos
/
Patología
/
Farmacología
/
Neoplasias Gástricas
/
Protocolos de Quimioterapia Combinada Antineoplásica
/
Leucovorina
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Curcumina
/
Línea Celular Tumoral
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2013
Tipo del documento:
Artículo
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