Determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>A quantitative method was developed for the determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC.</p><p><b>METHOD</b>The samples were separated by Alltima C18 column (4.6 mm x 250 mm, 5 microm) using methanol--0.05% phosphoric acid (6535) as a mobile phase, flow rate was 1.0 mL x min(-1) and wavelength was set at 202 nm.</p><p><b>RESULT</b>Tenuifolin was detected in both Chinese preparations. The number of theoretical plates calculated by tenuifolin peak was 2 500. The regression equation of tenuifolin was Y = 5.239 x 10(6) X-6.247 x 10(5) (r = 0.9994) and the liner range was 10-500 g x mL(-1). The average recovery of tenuifolin was 97.5% (RSD less than 3.0%). The LOD of tenuifolin was 5.50 g x mL(-1).</p><p><b>CONCLUSION</b>The method is sensitive, rapid and accurate.</p>