Simultaneous determination of three homoisoflavonoids in Ophiopogon japonicus by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 71-74, 2012.
Article
en Zh
| WPRIM
| ID: wpr-288643
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WPRO
ABSTRACT
Three homoisoflavonoids, 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3 PO4 (58:42) as the mobile phase, and the detection wavelength was set at 296 nm (0-14 min) and 275 nm (14-22 min). The mean recoveries of three homoisoflavonoids were 99.41%-99.86% (RSD 0.82%-1.05%). The linear response ranges of. 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 microg (r = 0.999 9), 0.153-0.918 microg (r = 0.999 9), and 0.270-1.620 microg (r = 0.999 9), respectively. This method was certified to be accurate and reliable and can be used for quality control of O. japonicus.
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Índice:
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Asunto principal:
Medicamentos Herbarios Chinos
/
Química
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Cromatografía Líquida de Alta Presión
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Ophiopogon
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Isoflavonas
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Article